نشریه دانشگاه علوم پزشکی البرز
سال نهم شماره 3 (تابستان 1399)

The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least time and cost. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is one of the novel methods of nucleic acid isolation. The aim of this study is to compare DNA extracted from Escherichia coli using two techniques including phenol-chloroform and magnetic nanoparticles.

In this study, Escherichia coli strain ATCC25922 was used to compare the two extraction methods. For genomic DNA extraction the phenol-chloroform methods as well as the technique of using bilayer SiO2/Fe3O4 magnetic nanoparticles were used. Both products were analyzed by nanodrop and following agarose gel electrophoresis.

The results of optical density (OD) evaluation with nanodrop spectrophotometer showed that the concentration of extracted DNA with conventional phenol-chloroform method and magnetic nanoparticle extraction technique were 761.4 µg/ml and 531 µg/ml respectively.

According to the obtained results by comparing the optical absorption of the two products and comparative evaluation with control groups in magnetic nanoparticle extraction method, also by comparing different factors such as the amount of time required, costs, ease of use, it can be concluded that due to the time-consuming and dangerous nature of the phenol-chloroform method, the using magnetic nanoparticles is an appropriated method for extracting the genomic content.

In modern world, attitudes of people are considered more important than their experiences and academic preparation. A positive attitude towards research is a key factor for success and progress in societies.

This study was psychometric assessment with 141 faculty member from Iran university of medical sciences. Initial instrument with 10 –items was translated to Persian after getting After permission from the developer of scale. Instrument validation procedures included impact item score, content validity index (CVI) and content validity ratio (CVR) of the judgment of 12 experts. Structural validity was assessed by exploratory factor analysis and internal reliability of the scale was determined by alpha Cronbach coefficient and with the test-retest method the external consistency of a test was evaluated.

Of the original 10 items, all of them based on impact item index over 1.5, CVR over 0.56, and CVI over 0.7 were kept in instrument. Due to exploratory factors analysis, 9 items remained in instrument. By considering a minimum eigenvalue of 1 for each factor, the two factors were extracted “For me research academic is positive” and “For me research academic is negative”. These factors were capable to predict 41.895% of the variances of total scale. Based on the confirmatory factor analysis, he extracted factors was approved.

The results of this study indicated the strength of the factor structure and reliability of an instrument for measuring faculty attitudes toward research scale. The results of study can assess faculty attitude in universities and other educational institutes.

In modern societies increasing tend to cosmetic surgery is worrying concern. This study was conducted to determine Affecting personality traits on tendency toward cosmetic surgeries among students of Alborz medical sciences university.

This study was cross sectional ( descriptive- analytical ) study. The statistical population was 1563 students from Alborz medical sciences university. The sample size was 352 students that were selected by s random sampling methods. NEO questionnaire (with 60 question) used for measuring the personality traits of the subjects. Data were analyzed using SPSS 10 software Chi-squared test was used for comparing frequencies.

Among Students who were interested or performed cosmetic surgery 32.14% were extraverted, 25% were conscientious and well-organized, 21.48% were openness, 14.28% percent were neurotic, 7.1% percent were Agreeableness to the cosmetic surgery.

The most effective personality trait on tendency toward cosmetic surgeries was extraversion and the least one, was agreeableness. The current study findings showed the impact of the psychological factors in request for cosmetic surgery. According to the findings of the study , the age of audiences and personality traits had a significant relation with tendency to the cosmetic surgery, Policymakers and university officials, with design and implement workshops in raising awareness of the students about the negative and positive dimensions of these surgeries, and preventing from the blind imitation.

Current study with the goal of reviewing The effectivness of imago training on marital intimacy between wife of addicted people in karaj Health center in (2018) was done.methodology of this study is semi experimental research in which pre-test,post-test design with control group was used. statitiscal society of this study includes 848 people and the sample size was 86 people(based on kokran formula calculations) they were put into two experimental and control groups (size of each group was 43)The assesments were used included VALTER AND TOMSON marital intimacy questionare the results were analyzed by spss software and based on single variable covariance. analysis results showed that there is a significant diffrence between the experimental group and control group in the post test in marital intimacy scores .the results of this study showed that imago training was effective to increase marital satisfaction.

 There are strong evidences that shows newly  nurses are not ready to enter the workplace.This issue may cause errors and the damages that can lead to many problems and adverse outcomes for patients. The preceptorship model for improving nursing education via action research approach is assessed in the study.

This study was conducted by action research method. Daily communications and monthly joint meetings were held from November 2015 to July 2016 for planning and management. Data were gathered via observation. The participants were 42 senior nursing students and 20 expert nurses. Both group participated in training sessions. In qualitative phase of study in-depth interview was conducted with 20 people including 15 students, 4 nurses and 1 nursing manager. In the quantitative phase all of the 42 students participated and their performance was assessed by OSCE. Also students’ satisfaction was examined by questionnaire. Three actions were developed and implemented. Qualitative data analysis was done by MAXQUDA software and SPSS was used for quantitative data analysis. 

Preceptors’ characteristics, clinical structure, communication between faculty and clinical environment were emerged from qualitative analysis.The most scores obtained from intravenous therapy and cardiopulmonary resuscitation stations, so that more than 55 percent of students were on the whole excellent and good. The lowest scores were reported in the history and nursing diagnoses and performances of 30 percent of the students were good, 42 percent 28 percent were moderate and poor respectively.

Our finding as a preliminary action research study are valubale implications to address challenges and also barriers to implement the preceptorship model in clinical education .

Diabetes is one of the most common chronic diseases and self-care and emotion play an important role in the control of the disease. The purpose of this study was to investigate the role of cognitive emotion regulation strategies in the relationship between self-care behaviors and severity of diabetes.

The present study is a descriptive cross-sectional study. The statistical population of the present study includes all type 2 diabetic patients referring to health centers, of which 108 patients (57 women, 51 men) were selected as a sample group by the purposeful sampling method. The participants completed the demographic questionnaire, the Cognitive Emotion Regulation Questionnaire (CERQ), and Summary of Diabetes Self-Care Activities (SDSCA).

The results of this study showed that the severity of the disease was negatively correlated with self-care and had a significant positive correlation with maladaptive cognitive emotion regulation strategies (P<0.01). The results of the hierarchical regression analysis indicated a moderating role for cognitive emotion regulation strategies in the relationship between self-care and the severity of type 2 diabetes.

According to the results, the severity of diabetes can be controlled through self-care promotion and adaptive cognitive emotion regulation strategies and these can be taken into account when designing therapeutic interventions and preventing diabetes.

MiRNAs are one of the most important genetic regulators that regulate more than 50 percent of the human genome. MiR-124-a-3 is a tumor suppressor miRNA which its expression dramatically reduced in prostate cancer tumor cells. Since miRNA binding to the transcript of target genes by seed sequence, any mutations and changes in this region could be effective on its performance and identify target genes.

Case-control study, used for assessment of association rs3405976 polymorphism in miR-124-a-3 gene with prostate cancer occurrence in 40 paraffin embedded tissue cancer samples and 5 ml control blood samples. After DNA extraction, the polymorphism determined with PCR-RFLP technique.

The GG genotype distribution in rs34059726 position of miR-124-a-3 gene showed in all patients and normal individuals. Blood group A with 42.2% have the most frequency in malignant patients and also, frequency of addicted patients reported 15% in malignant and 5% in benign patients.

rs34059726 polymorphism in miR-124-a-3 does not have associated with incidence of prostate cancer in the Gonbad kavous population and possibly other mechanisms are involved in decreased expression of miR-124-a-3 in prostate cancer in this population. In this population, addiction is a risk factor for prostate cancer and patients frequently have blood group A.

Primidone is effective in the treatment of epilepsy. In this research the effects of primidone on LH, FSH, testosterone hormones, body weight and testicular tissue in adult male rats, was studied.

In this experimental study, 50 adult male Wistar rats, weighing approximately 180-200 g were divided into 5 groups of 10 animals. Groups: control group, Sham group and treatment at doses of 20, 40, 60 mg/kg, orally for 42 days (every other day) respectively. At the end, after determining the animal's body weight, blood samples was collected from the heart. Hormone concentrations of LH, FSH and testosterone were measured by RIA.Their testis were removed and weighed to evaluate the histological changes after hematoxylin-eosin staining.

The body weight, testis weight and leydig and sertoli cells in all experimental groups showed no significant changes compared to the control group. The plasma levels of LH and FSH in all experimental groups increase significantly compared to the control group. The plasma level of testosterone Hormone and number of  spermatogonia,primary spermatocyte and spermatid in all experimental groups decrease significantly compared to the control group (P<0.05).

primidone reduced levels of testosterone and increase levels of LH and FSH hormones.

Restorative dentistry is an important branch of dentistry and therefore it is very important to train students that have all or the majority of the skills to start practice. The aim of this study was to compare new and old curricula of restorative lessons form the perspective of faculty members of selected Iranian universities.

The perspectives and viewpoints of the faculty members of dentistry schools in the cities of Tehran, Qazvin, Gilan, Shiraz, and Yasuj were collected using a valid and reliable researcher-made questionnaire. A demographic questionnaire was also applied to collect the characteristics of the participants. SPSS was used for data analysis and the Friedman test was administered to explore the strong and weak points.

The results showed a significant correlation between the new and the old curriculum (p<0.0014). The new curriculum had advantages like proceeding with and completing treatment stages according to a treatment plan and familiarization with dentists profession regulation in comprehensive dentistry course. The weak point of the new curriculum was introducing the topic of bonding in advanced theoretical remedial lessons, which seems to be early for students.

It seems that revising and modifying educational activities and content, including the curriculum of restorative lesson, are necessary in light of recent advances in dentistry. Therefore, curriculum studies may be helpful in this regard.

Elderly people have a disability in daily activities such as talking, eating and so on and needing prosthetic treatment. Patient satisfaction with complete dentures should be the primary goal of their treatment. The lack of awareness of the dentistry specialists is one of the reasons for the problems. The aim of this study was to assess the satisfaction of patients with dentures made by general practitioners, as well as experimental students and practitioners, in order to improve the success of prosthetic treatment and improve the quality of life of patients.

In this descriptive analytical cross-sectional study, 120 patients were randomly assigned to participate in the study by random sampling and participated in four groups A, B, C, D. Data analysis was performed using descriptive statistics, ANOVA and Tukey post hoc test. SPSS Version 18.0 (Inc., Chicago, IL, USA) software and R Version 3.2.2 software were used with dunn.test package. The significance level in this study was considered 0.05.

Significant differences were found between the groups (P <0.001) and Dentist overall satisfaction, Patients overall satisfaction, Esthetics, Ability to masticate, Ability to speak, Ease of cleaning, Comfort, Stability, Retention, and Functional limitation. . Also, there was a significant difference between the groups in the field of physical pain, psychological discomfort, physical disability, psychological disability, social disability (p <0.001).

There was a statistically significant difference between the basins in the study groups.

Human Herpes Viruses (HSV) contain eight pathogens. HSV is one of the most widespread viruses in the world and due to the simplicity of the way of transmission the number of people infected with the virus has increased in per year. The virus creates a wide range of diseases, including mucosal herpes infections and deadly herpes simplex encephalitis.Today, for the diagnosis of HSV infection several classic methods such as Morphological, Immunomorphologic, Virological and Serological methods, including Microscopic examination, Virus Culture, Western Blot and ELISA are used. But the speed, sensitivity and specificity of nucleic acid techniques (Molecular Technique) such as simple and modified PCR technique and Loop Mediated Isothermal Amplification (LAMP) have led them to become an attractive tool for diagnostic laboratories, and in particular the rapid identification of viral disease agents. In this review article, various methods for detecting herpes viruses were examined and compared.

Pressure ulcers are among the most serious skin and tissue disorders and put a heavy financial burden on healthcare systems. These ulcers break through the body’s first line of defense and can lead to severe consequences such as septicemia and death. Nowadays, most conventional treatments are very costly; therefore, the production of less expensive medications including herbal remedies is crucial. This study aimed to determine the healing effect of Dr Kamkar’s herbal cream in one to third stage Pressure Ulcer.

This single-blind randomized clinical trial was conducted on 65 patients aged 40-80 years with stage1- 3 Pressure ulcers at Imam Ali Hospital in Karaj.
The control group was treated with zinc oxide cream, while the intervention group was treated with Dr Kamkar’s herbal cream. Ulcers were measured according to the Pressure Ulcer Scale (PUSH) at the beginning of hospitalization and weekly for 8 weeks and the PUSH scores in the two groups were compered. Data analysis was carried out using SPSS version 23.

The results showed that in the intervention group the slop of decline in the PUSH score was greater in comparison to the control group. Regarding the area of the lesions, exudation and tissue regeneration, the levels of improvement in the intervention and control groups were 85% and 18%, respectively.

According to our data, a significant difference in the mean scores of the pressure ulcer was observed between the two groups (P ≤ 0.0001). It can be concluded that Dr Kamkar,s healing cream is effective in the treatment of stage one to third pressure ulcers.